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      當前位置:主頁 > 產品中心 > > 抗體/抗原 >Anti-Cyclin T1 antibody 抗細胞周期蛋白T1抗體

      Anti-Cyclin T1 antibody 抗細胞周期蛋白T1抗體

      簡要描述:Anti-Cyclin T1 antibody 抗細胞周期蛋白T1抗體
      貨號:ab27963
      廠家:Abcam公司
      規格:200ul
      保存溫度:-20℃ 和 2~8℃
      貨期:現貨2~5天,期貨2-3周

      • 產品型號:ab27963
      • 廠商性質:代理商
      • 更新時間:2025-02-12
      • 訪  問  量:1425
      詳細介紹

      咨詢:  (重慶) (貴陽)

      Anti-Cyclin T1 antibody 抗細胞周期蛋白T1抗體產品信息:

      • Product nameAnti-Cyclin T1 antibody
      • Description

        Sheep polyclonal to Cyclin T1

      • Tested applicationsWB, IP, EMSA
      • Species reactivityReacts with: Mouse, Horse, Human, Monkey

        Does not react with

        Fruit fly (Drosophila melanogaster)
      • Immunogen

        Recombinant fragment, corresponding to C terminal amino acids 291-726 of Human Cyclin T1

      • General notes

         

        ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.

      Properties

      • FormLiquid
      • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
      • PurityWhole antiserum
      • Primary antibody notes ab27963 will also recognise truncated forms of cyclin T1 which are frequently produced by proteolysis during sample preparation.
      • Clonality Polyclonal
      • IsotypeIgG
      • Application notesEMSA: Use at an assay dependent dilution PMID 17576689.
        IP: Use at an assay dependent dilution.
        WB: 1/1000. Detects a band of approximay 87 kDa (predicted molecular weight: 81 kDa).

        Not yet tested in other applications.
        Optimal dilutions/concentrations should be determined by the end user.
      • Target

      • FunctionRegulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.
      • Tissue specificityUbiquitously expressed.
      • Sequence similaritiesBelongs to the cyclin family. Cyclin C subfamily.
      • Cellular localizationNucleus.

        Anti-Cyclin T1 antibody images

        Western blot - Cyclin T1 antibody (ab27963)

        Western blot - Cyclin T1 antibody (ab27963)

        All lanes : Anti-Cyclin T1 antibody (ab27963) at 1/1000 dilution
        Lane 1 : HeLa whole cell lysate
        Lane 2 : HeLa cell nuclear lysate
        Lane 3 : monkey Vero cell lysate
        Lane 4 : mouse kidney cell lysate
        Lane 5 : Drosophila S2 cell lysate
        Lane 6 : horse dermal cell lysate
        Secondary
        donkey anti-sheep peroxidase conjugate at 1/20000 dilution
        Predicted band size : 81 kDa
        Observed band size : 87 kDa

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