人Caspase-3 FlowCytomix Simplex半胱天冬酶檢測試劑盒

英文名稱：Human Caspase 3 FlowCytomix Simplex
中文名稱：人Caspase-3 FlowCytomix Simplex檢測試劑盒

Human Caspase 3 FlowCytomix Simplex 產(chǎn)品介紹：
FlowCytomix Simplex試劑盒設(shè)計(jì)用于在流式細(xì)胞儀上免疫測定人Caspase-3 （半胱天冬酶-3）。
與FlowCytomix Human Basic Kit （cat. BMS8420FF）一起，它可用于單獨(dú)檢測Caspase-3或者與其它Simplex kits一起使用檢測不同裂解物。

Caspases （半胱天冬酶） 是天冬氨酸特異性半胱氨酸蛋白酶，如激酶一樣可以進(jìn)行級聯(lián)反應(yīng)。半胱天冬酶存在于所有細(xì)胞中，這些蛋白酶募集形成低聚體可致其激活并伴隨自我蛋白酶解。有活性的半胱天冬酶可以激活額外半光天冬酶產(chǎn)生級聯(lián)反應(yīng)，切割決定細(xì)胞存活的蛋白質(zhì)。
這種級聯(lián)信號通路zui終結(jié)果是稱為程序性細(xì)胞死亡的細(xì)胞凋亡。半胱天冬酶亞群充當(dāng)引發(fā)器和效應(yīng)器。Caspase-3特異性切割底物蛋白中天冬氨酸殘基羧基 端。活性Caspase-3有兩個(gè)活性位點(diǎn)，包含兩個(gè)相同分子量（～20 kDa）大亞基和兩個(gè)小亞基（～10 kDa），它們來自兩個(gè)前體Caspase-3多肽。Caspase-3可被其它半胱天冬酶水解激活。 

大小亞基都有助于底物結(jié)合和催化?？梢耘c底物共價(jià)結(jié)合活性位點(diǎn)位于大亞基C末端附近。Caspase-3雙重對稱，兩個(gè)活性位點(diǎn)口袋分別位于對稱軸 兩端。Caspase-3，同caspase 8 and 9都處在細(xì)胞凋亡路徑關(guān)鍵部位。Caspase-3可以增強(qiáng)caspase 8 and 9起始信號致使細(xì)胞凋亡裂解。

 Description: This FlowCytomix Simplex Kit is designed for the measurement of Human Caspase-3 in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect Caspase-3 alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.

This kit contains bead population B9.

Caspases are a family of aspartate-specific cysteine proteases that act in a step-wise signaling manner like kinases.
Caspases are present in all cells, recruitment of these proteases to oligomerized receptors leads to activation accompanied by autoproteolytic cleavage. Active caspases can proteolyze additional caspases generating a caspase cascade that cleaves proteins critical for cell survival. The final outcome of this signaling pathway is a form of controlled cell death termed apoptosis. The subgroup of caspases involved in apoptosis is called initiators or effectors. Caspase-3 cleaves substrate at the carboxyl terminus of aspartate residues. Active caspase-3 has two active sites and consists of two identical large (~ 20 kDa) and two identical small (~ 10 kDa) subunits that are derived from two precursor caspase-3 polypeptides. Caspase-3 is proteolytically activated by other caspases.

Both subunits contribute to substrate binding and catalysis. The active site cysteine that covalently binds the substrate is located near the C-terminus of the large subunit. Active caspase-3 has two-fold symmetry, two active site pockets each residing on an opposite side. Caspase-3, together with caspases 8 and 9, is situated at pivotal junctions in apoptotic pathways. Caspase-3 appears to amplify caspase 8 and 9 initiation signals into complete commitment to apoptotic disassembly.

參數(shù)：
? 反應(yīng)：人
? 樣品體積：25 µL
? 適合樣本類型：細(xì)胞裂解物
? 靈敏度：20 pg/mL
? 標(biāo)準(zhǔn)曲線范圍：82 – 60,000 pg/mL
? 成份：熒光珠1瓶（20倍濃縮），175 µl （涂有人Caspase-3單抗，具有Bead Population B9）；人Caspase-3標(biāo)準(zhǔn)物2瓶（凍干），1.2 µg/ml （重配制）；蛋白生物素結(jié)合物1瓶（20倍濃縮），350 µl （人Caspase-3多抗）；1瓶細(xì)胞裂解液濃縮物（10倍濃縮），15ml
? 應(yīng)用：多重免疫檢測